real-timePCR相关论文
番茄灰霉病是番茄生产上发生普遍、为害严重的气传病害,严重影响番茄的产量和质量。长期大量的施用化学农药容易引起病原菌抗药性......
利用逆转录重组酶介导的等温扩增技术（Reverse transcription-recombinase aided amplification, RT-RAA）与RT-RAA单链荧光探针结合，......
根据猪细环病毒2型（TTSuV2）基因非编码区保守序列设计了1对特异性引物,构建含有TTSuV2基因片段的重组质粒,以系列稀释后的重组质粒作......
为探讨旋毛虫预感染对小鼠急性脊髓损伤（spinal cord injury,SCI）的干预效果，选取40只C57BL/6雌性小鼠随机分为4组进行实验:A组（假手术......
期刊
由葡萄生单轴霉（Plasmopara viticola）侵染引起的葡萄霜霉病是世界范围内葡萄生产中最严重的病害之一,也是宁夏贺兰山东麓地区酿酒葡......
核因子NF-κB是细胞内重要的转录因子,其普遍存在于真核细胞中,它参与细胞的增殖分化,但是其过度活化将会引起免疫、炎症反应,并与......
学位
干旱、高盐、冷热等外界环境,严重的影响了植物的生长及产量。甘氨酸甜菜碱（简称甜菜碱）作为季铵类化合物,它被认为是很好的渗透胁迫......
探讨在Hepa1-6细胞中过表达、敲低Tmem176a基因对脂质代谢途径的影响.将构建成功的Tmem176a慢病毒过表达载体、siRNA分别转染进Hep......
【背景】Acinetobacter sp. Tust-DM21 （GenBank登录号KX390866）是本实验室前期从渤海湾海洋石油勘探船废油收集区采集的水油混合样......
目的 了解甘肃省白银市发热伴出疹症候群（RFIs）部分病原流行病学特征，为其预防和诊疗提供科学依据。方法 收集2018—2020年白银市稍点......
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烟草根黑腐病是烟草的一种严重土传病害,是由根串珠霉菌[Thielaviopsis basicola]为害根部引起的。此外,烟草根黑腐病还可以与烟草......
研究目的:本研究在海南岛发现了一种新型罗非鱼细小病毒（Novel tilapia parvovirus HMU-HKU）,对其进行全基因组扩增、遗传进化分析、......
目的:1.检测USP39蛋白和基因在乳腺癌组织中的表达水平。2.分析USP39蛋白和基因表达与乳腺癌临床病理指标的关系。方法:1.选取68例......
结核病是由结核分枝杆菌感染导致的一种古老的传染性疾病。结核病传播是全球性的公共卫生问题,中国是30个结核病高负担国家之一,而......
Methods and parameters of melting curve analysis for identification of Leishmania species: A scoping
Leishmaniasis is a set of diseases with a worldwide distribution that affects mainly economically underprivileged popula......
Herba Anoectochili is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the simila......
Development of a Real-time PCR Assay for the Detection and Quantification of Fish Bacterial Pathogen
The incidence of bacterial pathogens and their caused diseases has indicated the need for a reliable and rapid detection......
Analysis of gene expression profile in pollen development of recessive genic male sterile Brassica n
Male sterility in a near-isogenic line S45 AB with 25 generations of sub-crossing, is controlled by two pairs of duplica......
本试验依据烟草根黑腐病菌[T.basicola]与其它真菌在的ITS序列上的差异设计了一对特异性引物TbF/TbR,结果表明Phytophthora parasi......
玉米大斑病是由玉米大斑病菌(Setosphaeria turcica)引起的玉米叶部重要病害,在流行年份常造成严重的经济损失。许多植物病原真菌......
黄曲霉毒素是一类主要由黄曲霉和寄生曲霉产生的具有致畸致癌作用的次级代谢产物，能污染多种食品和饲料，严重危害人畜健康，造成巨大经......
Identification of Campylobacter jejuni and determination of point mutations associated with macrolid
The aim of the study was to develop a multiplex real-time PCR method to identify Campylobacter jejuni containing mutatio......
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Alteration of gene expression profile in the root of diploid wild Arachis duranensis inoculated with
Bacterial wilt caused by Ralstonia solanacearum is a serious disease of peanut (Arachis hypogaea L.) in China.The mechan......
Co-inoculation with porcine reproductive and respiratory syndrome virus and Bordetella bronchiseptic
Swine respiratory disease is generally considered a multifactorial problem caused by a combination of viral and bacteria......
田鼠巴贝斯虫(Babesia.microti)是一种寄生于动物红细胞的顶复门原虫，它可以通过蜱进行传播.至今已有数千例人感染巴贝斯虫病例报道......
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根据三角帆蚌的转录组数据,运用RACE PCR 的方法扩增出一个串联重复半乳糖凝集素基因,命名为Hc-galectin.克隆得到的基因全长为196......
Isolation, Identification of V.dahliae and a Molecular Method for Determining of Verticillium Wilt R
Studies on Chinese cabbage diseases focused on downy mildew,soft rot and virus disease,however a new Verticillium wilt d......
Decreased microbial diversity and Lactobacillus group in the intestine of geriatric giant pandas (Ai
It has been established beyond doubt that giant panda genome lacks lignin-degrading related enzyme,gastrointestinal micr......
Characterization of the cellulolytic bacteria communities along the gastrointestinal tract of Chines
A balanced gastrointestinal microbial ecosystem is crucial for the health and growth of animals.In the gastrointestinal ......
近年来高迁移率族蛋白B1(high mobility group B1 protein,HMGB1)的生物学功能研究成为国内外科学家研究的热点。本研究根据家禽鸡......
于2014年3月在成都动物园收集新鲜稀软粪便7份(编号DF1～DF7)、正常粪便7份(编号NF1～NF7)，利用变形梯度凝胶电泳(DGGE)和实时荧光定量(......
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从250日龄种鸡群中选取产蛋期、就巢期和恢复期各20只，屠宰取下丘脑、垂体、输卵管和卵巢，应用Real-time PCR检测GnRH基因在各组织中......
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为评估即食虾中混合菌群存在下的危害,建立低温下(4℃和10℃)即食虾中副溶血性弧菌与单增李斯特菌共同存在下的分子预测模型.将副......
Development of In situ hybridization and real-time PCR assays for the detection of Hepatospora erioc
A microsporidian parasite,Hepatospora eriocheir,is an emerging pathogen for the Chinese mitten crab Eriocheir sinensis.C......
秦艽(Gentiana macrophylla Pall.)为龙胆科(Gentianaceae)龙胆属(Gentiana)秦艽组多年生草本植物，国家重点保护的野生药材之一，主治......
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Synergistic effects and related bioactive mechanism of Potentilla fruticosa L.leaves combined with G
Ethnopharmacological relevance: Potentilla fruticosa,also called "Jinlaomei" and "Gesanghua",is widely used as folk ......
Molecular detection and quantification of nifH gene sequences in the rhizosphere of Oat(Avena sativa
[Objective]Biological nitrogen fixation(BNF)is an important source of nitrogen input in many natural ecosystems.Better e......
NAC是植物所特有的一类转录因子,参与植物生长发育、形态建成以及生物、非生物胁迫反应的抗逆过程.相比于拟南芥、水稻、小麦、大......
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谷锈病是谷子生产上的主要病害,流行年份植株倒伏、颗粒无收,严重影响着谷子的稳产和高产.筛选抗锈相关基因对于揭示抗锈机理意义......
Detection of Cyprinid Herpesvirus 2 Genome from Peripheral Blood Cells of Silver Crucian Carp Carass
Epidemics caused by Cyprinid herpesvirus 2(CyHV-2)in domestic cyprinid species have been reported in both European and A......
[Objective ]The objectives of this study are to clone goat PHLDA2(pleckstrin homology-like domain,family A,member 2),to ......
研究獭兔腹泻发生后盲肠菌群结构的变化情况,为微生态防治断兔奶腹泻提供理论基础.分别提取5只健康和腹泻獭兔盲肠内容物总DNA,应......
Additional Pyrite or Chalcopyrite Alters the Microbial Community Diversity, Composition and Function
Extraction of zinc fiom sphalerite using bio-hydrometallurgical technologies has become more and more popular.However, l......
Metallothionein cDNA Cloning and Combined Effects of Cd2+,Zn2+ on Its Expression in Pinctada martens
Metallothionein (MT),a type of small-molecule protein and widely distributed in organisms,plays an important role in det......
红笛鲷(Lutjanus sanguineus)俗称红鱼、红曹鱼，是我国南方沿海主要的经济鱼类之一。然而随着水产养殖业的迅猛发展，疾病频繁暴发，给......
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Severe necrotic dermatitis in the combs of line 63 chickens is associated with MDV-induced immunosup
Marek s disease (MD), a lymphoproliferative disorder of domestic chickens is characterized by bursal-thymic atrophy and ......
目前国内己发现至少三种基因型的草鱼呼肠孤病毒(Grass Carp Reovirus,GCRV)存在,不同基因型之间GCRV编码蛋白存在较大差异.GCRV S......
